Autoscan Systems Pty. Ltd. :
Free AutoScope Demonstration CD.
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What is AutoScope?AutoScope is a program that performs image analysis and automatic blob detection. It stores object measurements such as area, perimeter, circularity and eccentricity, as well as image thumbnails in an MSAccess 2.0 database format. This enables users to simply sort, select and modify the classification of objects found extremely quickly, and produces summaries of results with much greater accuracy and confidence.
AutoScope has been designed to run with the Autoscan range of microscope stages and the Data Translation range of frame grabbers. The combination of software and hardware results in a powerful tool that can be used to automate laborious, time consuming, subjective and often error prone microscopy tasks.
The full power of AutoScope has to be seen to be believed!
And now the demonstration version of AutoScope allows you to try out the software without the need for cameras, frame grabber hardware, microscopes or Autoscan stages! All you need is a PC running Windows 98 SE or NT Version 4 or higher, and having a screen resolution of 1024 x 768 x 24-bit colour.
In real life, AutoScope uses the Autoscan stage to position a microscopic sample over a defined area of interest (AOI) with micron resolution. Each image of a field of view in the AOI is then captured using a CCD camera and frame grabber. Objects in the captured image are automatically located and segmented from the background and then passed to the feature measurement module. Objects with features that satisfy predefined criteria are then stored in a database. This process continues for the entire AOI.
Please contact us at Autoscan Systems for a free AutoScope demo CD. Our demonstration version of AutoScope allows you to try out our software without the need to purchase an Autoscan stage ! All you need is an IBM-compatible PC running MSWindows 98 SE or NT Version 4 or higher, and having a screen resolution of 1024 x 768 x 24-bit colour.
Please also check out our document download page for a copy of the user manual
Autoscope automatically:
Controls the movement of the Autoscan stage,
Acquires images of the microscope field-of-view using a camera attached to the microscope,
Processes the acquired image, identifying pre-defined objects,
Stores the identified objects in a database for later review, and
Provides manipulation and export of stored data for post-processing.
The first step in setting up AutoScope is to configure the program for the particular hardware used (items such as cameras and frame-grabbers), and to establish the "Magnification Calibration Definitions" which calculate the relationship between the actual size of an object being viewed (in microns), and its apparent size on the computer screen (in pixels).
The next step is to customise the program for a given microscopic laboratory application.
In each case, one or more "Object Definitions" are created, which tell the program how to recognise the objects sought. These instructions usually relate to attributes such as the typical range of areas, perimeter lengths, and a range of other user-selectable parameters.
Typically, the objects being examined are mounted on one or more standard glass microscope slides, each of which may contain one or more "Regions of Interest" (ROIs). The locations of the boundaries of each of these ROIs are set by the operator. Object definitions can be applied simultaneously to more than one ROI. Often, more than one slide may be searched for similar or related objects. Such groups of slides, which are related for some reason, are known as a "Batch". Once the objects have been defined, the slides determined and their ROI's mapped, this information is saved in a batch file and processing can commence.
Processing consists of scanning all the ROIs on all slides in a given batch. Processing detects all defined objects, determines their location and shape or other attributes, and establishes a database which contains all of these data items, as well as a "thumbnail" image of each object. After processing, this database can be used for subsequent re-location of any object, and sorting, filtering and post-processing of the data set.
The data set is created by AutoScope as an Microsoft Access v2.0 database file. Its contents can thus be exported to a wide range of statistical and other data processing or data presentation packages.
AutoScope has been developed to provide automation of the analysis of microscopic images while still giving users control and therefore confidence in the final results. AutoScope has grown a lot in the many person-years that have gone into its development. A lot of this growth can be attributed to the feedback we have received from Autoscan customers. Because we act on this feedback, our customers benefit and the product benefits by being continuously improved. AutoScope is a dynamic program that is designed to be flexible and can, and will, be improved to meet your specific needs. We will be more than happy to discuss possibilities, improvements or modifications with you.
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